DETECTION SYSTEMS

Feature

We developed a unique immunohistochemical
staining system called Universal Immuno-enzyme Polymer (UIP)
method. Our polymer-based detection system, N-Histofine® series utilizing UIP provides custeomrs both high sensitivity and stable results in immunohistochemical applications.
see the advantages

We offer polymer-based detection system specialized for animal tissue staining.
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For human tissue

For animal tissue

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Application Guide

Reference

■ Human Tissue Sections

Simple Stain MAX PO (MULTI)

  1. (1) Mokrỳ, et al. Versatility of immunohistochemical reactions: comprehensive survey of detection systems. Acta medica 1996 Apr 39:129-40
  2. (2) Yamada K, et al. In vitro assessment of antitumor immune responses using tumor antigen proteins produced by transgenic silkworms. J Mater
    Sci Mater Med. 2021 May 17;32(6):58. .
  3. (3) Rahman A, et al. Reduced Claudin-12 Expression Predicts Poor Prognosis in Cervical Cancer. Int J Mol Sci. 2021 Apr 6;22(7):3774.
  4. (4) Tanaka Y, et al. A Novel Therapeutic Target for Melanoma. Int J Mol Sci. 2021 Jan 19;22(2):976.
  5. (5) Matsumoto NM, et al. Gene Expression Profile of Isolated Dermal Vascular Endothelial Cells in Keloids. Front Cell Dev Biol. 2020 Jul 29;8:658.
  6. (6) Oriuchi N, et al. Possibility of cancer-stem-cell-targeted radioimmunotherapy for acute myelogenous leukemia using 211At-CXCR4
    monoclonal antibody. Sci Rep. 2020 Apr 22;10(1):6810.
  7. (7) Ichinokawa K, et al. Downregulated expression of human leukocyte antigen class I heavy chain is associated with poor prognosis in non-small
    -cell lung cancer. Oncol Lett. 2019 Jul;18(1):117-126.
  8. (8) Yazawa T, et al. Prognostic significance of β2-adrenergic receptor expression in non-small cell lung cancer. Am J Transl Res. 2016 Nov
    15;8(11):5059-5070.
  9. (9) Uchida T, et al. CUL2-mediated clearance of misfolded TDP-43 is paradoxically affected by VHL in oligodendrocytes in ALS. Sci Rep. 2016 Jan
    11;6:19118.
  10. (10) Xing T, et al. Immunity of fungal infections alleviated graft reject in liver transplantation compared with non-fungus recipients. Int J Clin
    Exp Pathol. 2015 Mar 1;8(3):2603-14.
  11. (11) Kaira K, et al. Relationship between CD147 and expression of amino acid transporters (LAT1 and ASCT2) in patients with pancreatic
    cancer. Am J Transl Res. 2015 Feb 15;7(2):356-63.
  12. (12) Toyoda M, et al. Prognostic significance of amino-acid transporter expression (LAT1, ASCT2, and xCT) in surgically resected tongue cancer.
    Br J Cancer. 2014 May 13;110(10):2506-13.
  13. (13) Bychkov A, et al. Patterns of FOXE1 expression in papillary thyroid carcinoma by immunohistochemistry. Thyroid. 2013 Jul;23(7):817-28.

Simple Stain MAX PO (M)

  1. (1) Mokrỳ, et al. Versatility of immunohistochemical reactions: comprehensive survey of detection systems. Acta medica 1996 Apr 39:129-40
  2. (2) Kaji S, et al. BCAS1-positive immature oligodendrocytes are affected by the α-synuclein-induced pathology of multiple system atrophy.
    Acta Neuropathol Commun. 2020 Jul 29;8(1):120.
  3. (3) Kobayashi S, et al. Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and
    intranuclear cytoplasmic inclusions in lung adenocarcinoma. Oncol Rep. 2019 Jan;41(1):133-142.
  4. (4) Kudo I, et al. Particular gene upregulation and p53 heterogeneous expression in TP53-mutated maxillary carcinoma. Oncol Lett. 2017
    Oct;14(4):4633-4640.
  5. (5) Shimura T, et al. MIB-1 labeling index, Ki-67, is an indicator of invasive intraductal papillary mucinous neoplasm. Mol Clin Oncol. 2016
    Aug;5(2):317-322.
  6. (6) Ishiwata T, et al. Enhanced expression of fibroblast growth factor receptor 2 IIIc promotes human pancreatic cancer cell proliferation. Am J
    Pathol. 2012 May;180(5):1928-41.
  7. (7) Tsuji S, et al. Secretion of intelectin-1 from malignant pleural mesothelioma into pleural effusion. Br J Cancer. 2010 Aug
    10;103(4):517-23.
  8. (8) Tanioka Y, et al. Matrix metalloproteinase-7 and matrix metalloproteinase-9 are associated with unfavourable prognosis in superficial
    oesophageal cancer. Br J Cancer. 2003 Dec 1;89(11):2116-21.

Simple Stain MAX PO (R)

  1. (1) Mokrỳ, et al. Versatility of immunohistochemical reactions: comprehensive survey of detection systems. Acta medica 1996 Apr 39:129-40.
  2. (2) Sudo S, et al. Cisplatin-induced programmed cell death ligand-2 expression is associated with metastasis ability in oral squamous cell
    carcinoma. Cancer Sci. 2020 Apr;111(4):1113-1123.
  3. (3) Kudo I, et al. Particular gene upregulation and p53 heterogeneous expression in TP53-mutated maxillary carcinoma. Oncol Lett. 2017
    Oct;14(4):4633-4640.
  4. (4) Ishiwata T, et al. Enhanced expression of fibroblast growth factor receptor 2 IIIc promotes human pancreatic cancer cell proliferation. Am J
    Pathol. 2012 May;180(5):1928-41.
  5. (5) Tsuji S, et al. Secretion of intelectin-1 from malignant pleural mesothelioma into pleural effusion. Br J Cancer. 2010 Aug
    10;103(4):517-23.
  6. (6) Yokota N, et al. Self-production of tissue factor-coagulation factor VII complex by ovarian cancer cells. Br J Cancer. 2009 Dec
    15;101(12):2023-9.

■ Mouse and Rat tissue Sections

  1. (1) Ezure, K., et al: Glycine Is Used as a Transmitter by Decrementing Expiratory Neurons of the Ventrolateral Medulla in the Rat. The Journal of
    Neuroscience 23: 8941– 8948, 2003.
  2. (2) Kitada, M., et al: Translocation of Glomerular p47phox and p67phox by Protein Kinase C-β Activation Is Required for Oxidative Stress in Diabetic
    Nephropathy. Diabetes 52: 2603–2614, 2003.
  3. (3) Matsuyoshi, H., et al: Enhanced Priming of Antigen-Specific CTLs In Vivo by Embryonic Stem Cell-Derived Dendritic Cells Expressing Chemokine
    Along with Antigenic Protein: Application to Antitumor Vaccination. The Journal of Immunology 172: 776-786, 2004.
  4. (4) Noiri E., et al: Oxidative and nitrosative stress in acute renal ischemia. Am J Physiol Renal Physiol. 281: 948-957, 2001.
  5. (5) Kazuyo, M., et al: Innate production of TH2 cytokines by adipose tissue-associated c-Kit+Sca-1+ lymphoid cells. NATURE.
    doi:10.1038/nature08636,2009.
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