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PRIMARY ANTIBODIES

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N-Histofine® ALK Detection kit/ALK Control Slides

The ALK gene was identified in 1994 as a gene fused to the nucleophosmin (NPM) gene in anaplastic large-cell lymphoma (ALCL) with t(2;5)(p23;q35) translocation. This gene is located at 2p23 and encodes for a receptor-type tyrosine kinase, which belongs to the insulin receptor family. The ALK protein have a kinase domain in its intracellular domain, and its function is associated with the promotion of cell growth and inhibition of apoptosis. Subsequently, the ALK gene has been reported to form ALK fusion genes fused with ATIC, CLTC, MSN, TPM3, TPM4, TFG, MYH9 and ALO17 genes in ALCL and also ATIC, CARS, CLTC, DCTN1, TPM3, TPM4, PPFIBP1, RANBP2 and SEC31L1 genes in inflammatory myofibroblastic tumor (IMT). The proteins produced from these fusion genes are constantly activated by forming dimers and led to cancerous change. Recently, other ALK fusion genes with EML4 gene, KIF5B gene or KCL1 gene in non-small cell carcinoma of lung, SEC31A gene or SQSTM1 gene in ALK-positive large B-cell lymphoma and VCL gene in renal cell cancer have been also reported.

ALKDetection Kit

ALK control slide

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Product Name	Slides	Code
N-Histofine® ALK　Detection　Kit	20 tests	417071F
Vial NO.1: Blocking Reagent Vial NO.2: Primary Antibody Vial NO.3: Negative Control Vial NO.4: Bridge Reagent Vial NO.5: Peroxidase Labeled Empower Reagent Vial NO.6: Chromogen Substrate Vial NO.7: Substrate Buffer Solution Vial NO.8: Chromogen Reagent Vial NO.9: ALK Antigen Retrieval Solution A Vial NO.10: ALK Antigen Retrieval Solution B
N-Histofine® ALK Control Slides	5 slides	417081F
Each slide contains two formalin fixed paraffin embedded cell lines, NCI-H2228 (+: Positive) and SK-BR-3 (-: Negative).

Reference

ALK Detection Kit

(1) Shiota M., et al: Hyperphosphorylation of a novel 80 kDa protein-tyrosine kinase similar to Ltk in a human Ki-1 lymphoma cell line, AMS3.
Oncogene 9: 1567-1574, 1994
(2) Morris SW., et al: Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin’s lymphoma. Science. 263: 1281-1284, 1994
(3) Takeuchi K., et al: KIF5B-ALK, a novel fusion oncokinase identified by an immunohistochemistry-based diagnostic system for ALK-positive lung cancer. Clin Cancer Res. 15(9): 3143-3149, 2009
(4) Takeuchi K., et al: Pulmonary inflammatory myofibroblastic tumor expressing a novel fusion, PPFIBP1-ALK: reappraisal of anti-ALK
immunohistochemistry as a tool for novel ALK-fusion identification. Clin Cancer Res. 17(10): 3341-3348, 2011
(5) Sugawara E., et al: Identification of Anaplastic Lymphoma Kinase Fusions in Renal Cancer. Cancer, 2012

Sialylated HEG1（SKM9-2）HC

This antibody reacts with human sialylated HEG1 protein⁽¹⁾. Sialylated HEG1 is a mucin-like membrane protein with many sialylated O-linked glycans linked to the serine/threonine rich region in the extracellular domain. The antibody recognizes the sialylated 893 SKSPSLVSLPT903 sequence in the serine/threonine rich region⁽²⁾. Although the function of sialylated HEG1 has not yet been investigated, it is expressed especially in the cell membrane and cytoplasm of malignant mesothelioma cells. In malignant mesothelioma, expression was found with ~92% sensitivity and ~99% specificity, which is better performance than that provided by other major malignant mesothelioma diagnostic markers⁽¹⁾ ⁽³⁾ ⁽⁴⁾. In normal tissues, very low expression of sialylated HEG1 is observed, but a few positive cases may be found in capillary endothelium or reactive mesothelial cells.

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Further manufacturing

Product Name	Slides	Code	Instruction	Safety Data Sheet
Sialylated HEG1（SKM9-2）HC	1ml	498331F

Reference

HEG1

(1) Tsuji S, et al. HEG1 is a novel mucin-like membrane protein that serves as a diagnostic and therapeutic target for malignant mesothelioma. Sci Rep. 2017 Mar 31;7:45768.
(2) Matsuura R, et al. Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1. Sci Rep. 2018 Sep 24;8(1):14251.
(3) Hiroshima K, et al. HEG1, BAP1, and MTAP are useful in cytologic diagnosis of malignant mesothelioma with effusion. Diagn Cytopathol. 2020 May 22.
(4) Naso JR, et al. HEG1 Is a Highly Specific and Sensitive Marker of Epithelioid Malignant Mesothelioma. Am J Surg Pathol. 2020 Aug;44(8):1143-1148.