Detection Systems For Animal tissue｜Immunohistochemistry

DETECTION SYSTEMS
For Animal tissue

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When immunohistochemical detection systems for human tissue sections are used to stain mouse and rat tissue sections, background staining may occur due to reactions with mouse and rat endogenous immunoglobulins in the tissue. Therefore, we developed an immunohistochemical detection system for staining of mouse and rat tissue sections.

A report comparing the background staining properties of the detection system on mouse, rat, and human tissue sections is presented.

For Mouse

For Rat

Advantage

For Mouse

With mouse primary antibodies

N -Histofine® MOUSESTAIN KIT is the mouse on mouse system. It is designed specifically to allow immunohistochemical staining with a mouse primary antibody on formalin-fixed paraffin-embedded mouse tissue sections. This kit consists of Blocking reagent A, Blocking reagent B, and Simple Stain™ Mouse Max PO (M) which is the labeled polymer prepared by combining amino acid polymer with multiple molecules of peroxidase and goat anti-mouse Ig which is reduced to Fab’ fragment. To eliminate background staining, this kit uses Blocking reagent A and Blocking reagent B.

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Product Name	Slides	Volume	Code	For use with	Instruction
N-Histofine® MOUSESTAIN KIT	50	6ml x 3	414321F	Mouse primary antibody
N-Histofine® MOUSESTAIN KIT	500	17ml x 9	414322F	Mouse primary antibody

With rabbit, goat or rat primary antibodies

N-Histofine® Simple Stain™ Mouse MAX PO is a detection reagent designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded mouse tissue sections. It is the labeled polymer prepared by combining amino acid polymers with peroxidase(PO) and secondary antibody which is reduced to Fab’ fragment. To eliminate background staining, solid-phase adsorption of secondary antibody is carried out with mouse serum.

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Product Name	Slides	Volume	Code	For use with
N-Histofine® Simple Stain™ Mouse MAX PO(R)	170	17ml	414341F	Rabbit primary antibodies
N-Histofine® Simple Stain™ Mouse MAX PO(G)	170	17ml	414351F	Rabbit primary antibodies
N-Histofine® Simple Stain™ Mouse MAX PO(Rat)	170	17ml	414311F	Rabbit primary antibodies

For Rat

With mouse, rabbit, or goat primary antibodies

N-Histofine® Simple Stain™ Rat MAX PO is a detection reagent designed specifically to allow immnuhistochemical staining on formalin-fixed paraffin-embedded rat tissue sections. It is the labeled polymer prepared by combining amino acid polymers with multiple molecules of peroxidase(PO) and secondary antibody which is reduced to Fab’ fragment. To eliminate background staining, solid-phase adsorption of secondary antibody is conducted with rat, human, dog, pig and bovine serum.

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Product Name	Slides	Volume	Code	For use with
N-Histofine® Simple Stain™ Rat Max PO(MULTI)	170	17ml	414191F	Mouse and rabbit primary antibodies
N-Histofine® Simple Stain™ Rat Max PO(M)	170	17ml	414171F	Mouse primary antibodies
N-Histofine® Simple Stain™ Rat Max PO(R)	170	17ml	414181F	Rabbit primary antibodies
N-Histofine® Simple Stain™ Rat Max PO(G)	170	17ml	414331F	Goat primary antibodies

Advantage

When the immunohistochemical detection systems for human tissue sections are used for staining on mouse and rat tissue sections, the background staining may be caused due to such reactivity with endogenous immunoglobulins of mouse and rat in the tissue. So that the immunohistochemical detection systems designed for staining on mouse and rat tissue sections were developed.

See the techincal report

■ Technical Report

Fig.1 Mouse tissue sections

N-Histofine®
Simple Stain™ Mouse MAX PO(Rat)

normal colon

normal spleen

N-Histofine®
Simple Stain™ MAX PO(M)

normal colon

normal spleen

Fig.2 Rat tissue sections

N-Histofine®
Simple Stain™ Rat MAX PO(M)

normal colon

Background staining is NOT observed.

N-Histofine®
Simple Stain™ MAX PO(M)

normal colon

Background staining in plasma cells is observed.

Reference

■ Mouse and Rat tissue Sections

(1) Ezure, K., et al: Glycine Is Used as a Transmitter by Decrementing Expiratory Neurons of the Ventrolateral Medulla in the Rat. The Journal of Neuroscience 23: 8941– 8948, 2003.
(2) Kitada, M., et al: Translocation of Glomerular p47phox and p67phox by Protein Kinase C-β Activation Is Required for Oxidative Stress in Diabetic Nephropathy. Diabetes 52: 2603–2614, 2003.
(3) Matsuyoshi, H., et al: Enhanced Priming of Antigen-Specific CTLs In Vivo by Embryonic Stem Cell-Derived Dendritic Cells Expressing Chemokine Along with Antigenic Protein: Application to Antitumor Vaccination. The Journal of Immunology 172: 776-786, 2004.
(4) Noiri E., et al: Oxidative and nitrosative stress in acute renal ischemia. Am J Physiol Renal Physiol. 281: 948-957, 2001.
(5) Kazuyo, M., et al: Innate production of TH2 cytokines by adipose tissue-associated c-Kit+Sca-1+ lymphoid cells. NATURE. doi:10.1038/nature08636,2009.