Materials

– Formalin-fixed paraffin-embedded mouse tissue sections

– Formalin-fixed paraffin-embedded rat tissue sections

– -Histofine® Simple Stain Mouse MAX PO (Rat) :
a detection reagent designed for staining with a rat primary antibody on mouse tissue sections

– -Histofine® Simple Stain Rat MAX PO (M) :
a detection reagent designed for staining with a mouse primary antibody on rat tissue sections

– -Histofine® Simple Stain MAX PO (M) :
a detection reagent designed for staining with a mouse primary antibody on human tissue sections

Methods

To compare background staining between detection reagents, immunohistochemical staining on mouse tissue sections was performd with -Histofine® Simple Stain Mouse MAX PO (Rat) and -Histofine® Simple Stain MAX PO (M). (Fig.1)

To compare background staining between detection reagents, immunohistochemical staining on rat tissue sections was performed with -Histofine® Simple Stain Rat MAX PO (M) and
-Histofine® Simple Stain MAX PO (M). (Fig.2)

PBS was used instead of a primary antibody in order to identify the background staining caused by detection reagents. DAB solution was used for brown color development.

Steps of immunohistochemical staining